Clínica dental

Diseases Linked to Scaffold Proteins and Signaling | Science Lab

[título_original] – Nueva actualización 2023

Somos un pequeño grupo de apasionados profesionales de la odontología que han estado escribiendo artículos para el público durante más de 10 años. Nuestra misión es proporcionar información precisa y actualizada sobre la salud bucal para que las personas puedan tomar decisiones informadas sobre su atención dental.
Sabemos que una excelente salud bucal es esencial para la salud y el bienestar general, y estamos comprometidos a ayudar a nuestros lectores a lograr y mantener dientes y encías saludables.
csm GFP tagged zebrafish fin widefield THUNDER f67ef620ae

There is a significant amount of research focused on diseases which are caused by mutations in scaffold proteins, like Daple, that regulate crosstalk between G protein signaling and other growth promoting pathways [1,2]. Mutations in these proteins have been linked to abnormalities like hydrocephalus and several cancers [1,2]. Many cancer researchers investigate protein signaling in model organisms like zebrafish using genetically modified fluorescent strains with the hopes of gaining a better mechanistic understanding of how faulty signaling causes disease [1,2].

Challenges when imaging model organisms

One of the limitations when imaging model organisms is the autofluorescence seen in some of the zebrafish tissues and the time it takes to image them. Conventionally, most of the imaging is done using confocal microscopy to achieve adequate resolution [1,2]. This involves acquisition of z-stacks in multiple fluorescent channels and would take on average 10-15 minutes to acquire a confocal image.

Methods

Zebrafish fin tissue immunostained for GFP (yellow), actin (magenta), and nuclei (DAPI, cyan) was used for this study. Specimens were imaged with a THUNDER Imager Live Cell having a 20x, 0.8 NA (numerical aperture) objective. A z-stack of 162 slices every 0.5 µm (total of 80.25 µm) was obtained and processed using large volume (LVCC) or instant computational clearing (ICC) [3,4]. Images were acquired with speeds typical of widefield microscopy. Extended depth of field (EDoF) projections are shown.

Results

With the Thunder Imager Live Cell, the time to acquire a z-stack of 3 channels and 162 slices was just over 1 minute and 10 seconds, allowing a large increase in output compared to confocal microscopy. Additionally, by combining ICC and deconvolution, the background was greatly reduced and fine details more clearly revealed. THUNDER images with enhanced contrast and resolution are seen in comparison to raw widefield ones in figure 1 and 2.

Lea más publicaciones relacionadas [título_original] en la misma categoría

Somos un pequeño grupo de personas apasionadas que han estado escribiendo artículos dentales durante los últimos años. Creemos que una buena salud oral es la clave para una vida feliz y saludable. Nuestro objetivo es proporcionar información precisa y actualizada sobre todos los aspectos de la odontología para que nuestros lectores puedan tomar decisiones informadas sobre su salud bucal.
Sabemos que elegir un dentista o un tratamiento dental puede ser abrumador, pero esperamos que nuestros artículos ayuden a que el proceso sea un poco más fácil.
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